Characterization of chikusaku-eki and mokusaku-eki and its inhibitory effect on sapstaining fungal growth in laboratory scale

Chikusaku-eki and Mokusaku-eki are natural resources and acidic liquid by-products of bamboo and broad leaved trees charcoal burner. These products contain more than 200 ingredients, including phenols, poly phenols and acetic acids. These by-products were tested for their fungicidal activity against sapstaining fungi. There are no studies about the antifungal activity of Chikusaku-eki and Mokusaku-eki against wood staining fungi in the literature. According to the recent findings, this is the first report about the antifungal activity of Chikusaku-eki and Mokusaku-eki against wood staining fungi. These extracts were more effective against sapstaining fungi at minimum concentrations [0.10-1.0%] used in 2% [malt extract agar] medium. Three Chikusaku-eki [Chikusaku-eki-I, Chikusaku-eki-II and Chikusaku-eki-III] and 2 Mokusaku-eki [Mokusaku-eki-I, Mokusaku-eki-II] extracts were tested against 4 sapstaining fungal samples to evaluate the inhibition range on sapstaining fungal growth. Ophiostoma flexuosum, Ophiostoma tetropii, Ophiostoma polonicum and Ophiostoma ips were the sapstaining fungi used in this study against bamboo and wood extracts. The initial calibration of extracts was done by calculating the specific gravity, tar calculation and total organic content. The chloroform fractions of these extracts were analyzed by gas chromatography-mass spectrometry and both the extracts contained 2,6 dimethoxy phenol, dehydroacetic acid and 2,3,5 trimethoxytoluene. Results revealed that compounds of Chikusaku-eki and Mokusaku-eki markedly inhibited fungal growth at lower concentration. The Chikusaku-eki and Mokusaku-eki have both antifungal, antioxidant properties and a potential to be used as natural preservative in wood industries. Wood slice tests displayed the effective role of both extracts in laboratory level

A Case of Metastatic Malignant Melanoma / 대한피부과학회지

We present a case of metastatic mailgnant melanoma developed in the 40 year- old male patient. Six month prior to visit to our department of dermatology, the patient found an egg sized mass covered with normal skin on his right inguinal area, which showed typical findings of the metastatic malignant melanoma histopathologically. The primary malignant melanoma on the right shin was removed by wide surgical excision at 18 months prior to visit to our department of dermatology.